U-2932-CAS9 cell line

location： Home > Products > Cas9 Stable Cell Lines > U-2932-CAS9

U-2932-CAS9 Order

Catalog#

YC-C059-Cas9-H

Size

1*10^6

Transfection Culture Medium

Transfection Culture Medium--Suspension Cells

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

Cas9 Stable Cell Lines

cell name

U-2932-CAS9

Cell Morphology

Human Lymphocyte-like, suspension

Passage ratio

1：2-1：4

Species

Human

Expression gene

Cas9

Resistance gene

hygro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90%RPMI-1640+10%FBS

Description

Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the U-2932 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a U-2932-CAS9 cell line that can maintain long-term, stable and high expression.

Cutting Efficiency Validation

U-2932-CAS9 cell line Product Validation Data（RT-QPCR）

Note: The above figure shows the sequencing peaks of the U-2932-CAS9 stable cell pool which is electroporated by BTK gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:U-2932			STR Info (Cell bank) Cell Line:U-2932
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	12	12		12	12
D13S317	11	11		11	11
D7S820	9	13		9	13
D16S539	12	12		12	12
VWA	14	17		14	17
TH01	9.3	9.3		9.3	9.3
AMEL	X	X		X	X
TPOX	11	12		11	12
CSF1PO	10	12		10	12
D12S391	18	20
FGA	22	24
D2S1338	22	23
D21S11	28	30
D18S51	15	15
D8S1179	13	14
D3S1358	15	16
D6S1043	18	18
PENTAE	13	14
D19S433	14	14
PENTAD	14	14
D1S1656	16	16.3

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
01
High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
02
High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
03

U-2932-CAS9

Catalog#

YC-C059-Cas9-H

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Related Popular Products

KO Cell Lines

BTK Knockout cell line (U-2932)
inquiry More details

Organism: Human

Gene Name: BTK

Gene ID: 695

Size: 1*10^6

Catalog#: YKO-H067

YKO-H067(U-2932)
ENPP2 Knockout cell line (U-2932)
inquiry More details

Organism: Human

Gene Name: ENPP2

Gene ID: 5168

Size: 1*10^6

Catalog#: YKO-H055

YKO-H055(U-2932)

Luciferase Stable Cell Lines

U-2932-Luc
inquiry More details

Catalog#: YC-C059-Luc-P

Size: 1*10^6

Instruction:

Wild-type Cell Lines

Human B Cell Lymphoma Cell Line(U-2932)
inquiry More details

Catalog#: YC-C059

Size: 1*10^6

Instruction:

Related Popular Products

KO Cell Lines

BTK Knockout cell line (U-2932)

Organism: Human

Gene Name: BTK

Gene ID: 695

Size: 1*10^6

Catalog#: YKO-H067

inquiry More details
ENPP2 Knockout cell line (U-2932)

Organism: Human

Gene Name: ENPP2

Gene ID: 5168

Size: 1*10^6

Catalog#: YKO-H055

inquiry More details

Luciferase Stable Cell Lines

U-2932-Luc

Price (USD): -

Catalog#: YC-C059-Luc-P

Size: 1*10^6

Instruction:

inquiry More details

Wild-type Cell Lines

Human B Cell Lymphoma Cell Line(U-2932)

Price (USD): -

Catalog#: YC-C059

Size: 1*10^6

Instruction:

inquiry More details

Subscribe Us

* E-mail：

Country/Region：

Name：

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.

U-2932-CAS9 cell line

Ubigene